No pharmacokinetic studies have been done in wildlife animals. The pharmacokinetics of midazolam in domestic animals shows variation between species and routes of administration.

In dogs, the elimination half-life is variable (0,46 hours following IV administration to 1,6 hours following IM administration) with a high clearance rate (10-28 ml/kg/min). In horses, the elimination half-life is 3,6 hours following IV administration and 6,8 hours following IM administration with a high clearance rate of 10,4 – 10,6 ml/min/kg. In sheep, the elimination half-life of midazolam following IV administration is 0,78 hours with a clearance of 21,2 ml/min/kg. Midazolam is metabolised in the liver and the metabolites are excreted in the urine. Elimination of midazolam occurs in a biphasic manner and consists of an initial rapid redistribution phase, followed by a slower terminal phase.

Aqueous midazolam preparations produce little tissue irritation when injected. Following injection into an environment of relatively neutral tissue pH, the chemical structure of midazolam shifts to a completely ring-closed form, which makes the medicine highly lipophilic. This lipophilic form is rapidly absorbed from the injection site and easily penetrates the CNS.